FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007813345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007813345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448024443
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG251541155.614451486523024TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCCCGTATG8406780.1876411015369534TruSeq Adapter, Index 7 (97% over 37bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG4645370.10368563752670074TruSeq Adapter, Index 9 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)