FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007815866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20736
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTGTA4972.396797839506173No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC2691.2972608024691359No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.8728780864197532No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.7378472222222222No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTC1210.5835262345679012No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1150.5545910493827161No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC980.47260802469135804No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.3809799382716049No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.3568672839506173No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG540.26041666666666663No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC520.2507716049382716No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC480.23148148148148145No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG460.2218364197530864No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC450.2170138888888889No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT440.21219135802469136No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT440.21219135802469136No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG430.20736882716049385No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.19290123456790123No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT390.1880787037037037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC390.1880787037037037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC380.18325617283950615No Hit
GTGAGAAGGTGAGGCCAGAAGTGGGGAGATGGGTAAGAAATGACTGCAAT370.17843364197530864No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA350.16878858024691357No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC350.16878858024691357No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC340.16396604938271603No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTGTAGA330.15914351851851852No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC330.15914351851851852No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT330.15914351851851852No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.15432098765432098No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG310.14949845679012344No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.14467592592592593No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC300.14467592592592593No Hit
CGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.1398533950617284No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC290.1398533950617284No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT290.1398533950617284No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG280.13503086419753085No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTG280.13503086419753085No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC280.13503086419753085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC260.1253858024691358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG260.1253858024691358No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC260.1253858024691358No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT250.12056327160493827No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA250.12056327160493827No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT250.12056327160493827No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC240.11574074074074073No Hit
CACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11574074074074073No Hit
CCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTT230.1109182098765432No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG220.10609567901234568No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC220.10609567901234568No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT220.10609567901234568No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT220.10609567901234568No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10609567901234568No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACG210.10127314814814814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)