FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007815868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13642
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTGTA4032.95411230024923No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC3072.250403166691101No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1401.0262424864389386No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1210.8869667204222255No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.7990030787274593No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC980.718369740507257No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTC880.6450667057616185No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.432487904999267No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.36651517372819237No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.30787274593168157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG400.2932121389825539No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC360.2638909250842985No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTGTAGA360.2638909250842985No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC360.2638909250842985No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC340.2492303181351708No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC330.24190001466060695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA320.23456971118604308No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG310.22723940771147924No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC310.22723940771147924No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC300.2199091042369154No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.21257880076235156No Hit
CAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCA280.2052484972877877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG260.19058789033866003No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.19058789033866003No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGTTCTTCGTG260.19058789033866003No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT240.17592728338953234No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT240.17592728338953234No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT230.1685969799149685No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA230.1685969799149685No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGATTTTTTTT220.16126667644040463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA220.16126667644040463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC220.16126667644040463No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC220.16126667644040463No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT210.15393637296584078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC210.15393637296584078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC200.14660606949127694No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT200.14660606949127694No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA200.14660606949127694No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1392757660167131No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC190.1392757660167131No Hit
CCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTT190.1392757660167131No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC180.13194546254214926No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT180.13194546254214926No Hit
CGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.1246151590675854No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG170.1246151590675854No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.1246151590675854No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC160.11728485559302154No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA150.1099545521184577No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA150.1099545521184577No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATACGT140.10262424864389386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC140.10262424864389386No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA140.10262424864389386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC140.10262424864389386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)