FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007815919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18209
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCT4112.257125597232138No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2351.2905705969575485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1971.081882585534626No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCG1861.0214728980174639No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1450.7963095172716789No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGG1150.6315558240430557No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.6315558240430557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1130.6205722444944808No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA720.3954088637486957No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG590.324015596682959No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC550.3020484375858092No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC530.29106485803723436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT480.2636059091657971No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT480.2636059091657971No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG480.2636059091657971No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG460.25262232961722225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT450.24713053984293482No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTAT420.2306551705200725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT420.2306551705200725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT410.22516338074578504No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT410.22516338074578504No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT370.20319622164863527No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.19221264210006042No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT350.19221264210006042No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT340.18672085232577296No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA320.17573727277719808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT320.17573727277719808No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA310.17024548300291065No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG300.1647536932286232No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG290.15926190345433577No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC290.15926190345433577No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCTCG280.15377011368004834No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTT270.1482783239057609No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC260.14278653413147344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG260.14278653413147344No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC260.14278653413147344No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.137294744357186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA240.13180295458289856No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG230.12631116480861113No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT220.12081937503432369No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.10983579548574882No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT200.10983579548574882No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.10434400571146137No Hit
ATTGCGCAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10434400571146137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)