FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007815923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25055
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTAGGACAATCT5482.1871881859908204No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG2781.109558970265416No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2721.085611654360407No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTAGGACAATCG2541.0137697066453801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1660.6625424067052484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1590.634603871482738No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.610656555577729No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTAGGAC1290.5148672919576931No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG850.3392536419876272No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA830.3312712033526242No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT770.30732388744761524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT770.30732388744761524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC730.29135901017760923No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC710.28337657154260626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT700.2793853522251048No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTAGGACAAT630.25144681700259425No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC570.22749950109758532No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG550.2195170624625823No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG550.2195170624625823No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT540.21552584314508083No Hit
ATTGCGCAATGGAAGTCGCGGGTAACCTGCATCTTCACAGGTACTAAAAT470.18758730792257033No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGT430.17162243065256436No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGT430.17162243065256436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG410.16363999201756135No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC410.16363999201756135No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGTAGGACAATCTCG400.15964877270005987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT400.15964877270005987No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.15964877270005987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT400.15964877270005987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA390.15565755338255838No Hit
ATTGCGCAATGAAAAAAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.15565755338255838No Hit
ATTGCGCAATGTAAAGGGAGGGAGGTGTATCGCTTGGGCTCAGGAGTTCG380.15166633406505686No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGT370.14767511474755537No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG370.14767511474755537No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT360.14368389543005386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT360.14368389543005386No Hit
ATTGCGCAATGTTGGACTTGGGGAAGTATATTGTAATTGTTTAATCTTAG360.14368389543005386No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC350.1396926761125524No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGT330.1317102374775494No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA330.1317102374775494No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC320.12771901816004788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC320.12771901816004788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG320.12771901816004788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC320.12771901816004788No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTT320.12771901816004788No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.12372779884254641No Hit
ATTGCGCAATGAAACCATAGGGATAACTTAGCACTCGATGCATTAGAGTG310.12372779884254641No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC300.11973657952504489No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGT290.11574536020754342No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA280.1117541408900419No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT280.1117541408900419No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA260.10377170225503891No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG260.10377170225503891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)