FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007815924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25055
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC5762.2989423268808618No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGGGTGTA3961.5805228497305928No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2551.0177609259628817No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA2120.8461384953103174No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.7303931351027738No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1740.6944721612452605No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGGGTGGA1580.6306126521652365No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGGGTGTT1520.6066653362602275No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGG1330.530832169227699No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGGGTGGT1070.42706046697266015No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.40311315106765117No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG970.3871482737976452No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.3671921772101377No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC900.3592097385751347No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG700.2793853522251048No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTACCGAGGGTG690.2753941329076033No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC660.2634204749550988No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG590.2354819397325883No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.2235082817800838No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA550.2195170624625823No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC510.2035521851925763No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC510.2035521851925763No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG480.19157852724007185No Hit
CAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCA480.19157852724007185No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT480.19157852724007185No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC480.19157852724007185No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTAC450.17960486928756736No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTAC430.17162243065256436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC420.16763121133506287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC420.16763121133506287No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC400.15964877270005987No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTAC400.15964877270005987No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA390.15565755338255838No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG380.15166633406505686No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC370.14767511474755537No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG360.14368389543005386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC360.14368389543005386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC360.14368389543005386No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG360.14368389543005386No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA340.13570145679505088No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGATTTTTTTT340.13570145679505088No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCA340.13570145679505088No Hit
CAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCA330.1317102374775494No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTAC320.12771901816004788No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT310.12372779884254641No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAACTAC300.11973657952504489No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.11973657952504489No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAA300.11973657952504489No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC290.11574536020754342No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC290.11574536020754342No Hit
CCCCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTT290.11574536020754342No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA290.11574536020754342No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA280.1117541408900419No Hit
CACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10776292157254042No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC260.10377170225503891No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC260.10377170225503891No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC260.10377170225503891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)