FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007815971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40646
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCT6051.4884613492102545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.9029178762977907No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCG3340.8217290754317769No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG2980.7331594744870343No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCG2290.5634010726762781No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.528957338975545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1890.4649904049598977No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1710.4207056044875265No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT1390.3419770703144221No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1340.32967573684987456No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.29277173645623183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG980.2411061359051321No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT930.22880480244058454No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT870.21404320228312748No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT840.20666240220439894No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC800.19682133543276092No Hit
ATTGCGCAATGACATTAGTGGGGACGTGCGGTTCTATTTTGTTGGTTTCT790.1943610687398514No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGAT730.17959946858239434No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC710.17467893519657532No Hit
ATTGCGCAATGGCGGGGATGGGTGCTTGGAACTGTTACACTTGCTAGCTT700.1722186685036658No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG690.16975840181075627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG690.16975840181075627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT680.16729813511784677No Hit
ATTGCGCAATGAGCTTGCTGGGGTGTGGGTTATCACTAATCCTACCAATT630.1549968016532992No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG630.1549968016532992No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC610.1500762682674802No Hit
ATTGCGCAATGTGCGTCCAGGGGTCATATGCTTGTCTCAAAGATTAAGCC590.14515573488166117No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG590.14515573488166117No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG580.14269546818875165No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC580.14269546818875165No Hit
ATTGCGCAATGCCTAGGTGGGTCATATGCTTGTCTCAAAGATTAAGCCAT540.1328544014171136No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT540.1328544014171136No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC530.1303941347242041No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT490.12055306795256605No Hit
ATTGCGCAATGTATGACTTGGGCTAAAAACCCCGACTTCAGGAAGGGGTG490.12055306795256605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA480.11809280125965654No Hit
ATTGCGCAATGGCTCTGCAGGGGTTAATAATTAAAAAGAAAAATAGGAAA470.11563253456674703No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG460.11317226787383754No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG450.11071200118092801No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC440.1082517344880185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT430.10579146779510899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC430.10579146779510899No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG430.10579146779510899No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA420.10333120110219947No Hit
ATTGCGCAATGGGGGAGTAGGGCAACTTGTACTGTGAAACTGCGAATGGC420.10333120110219947No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG420.10333120110219947No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC410.10087093440928996No Hit
ATTGCGCAATGTCTGTGGAGGGATACCCTAGTAGTCCAAGCAGACAATGA410.10087093440928996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)