FastQCFastQC Report
Tue 7 May 2024
EGAF00007815972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007815972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40646
Total Bases6.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGTGTGTA4691.1538650789745608No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.851252275746691No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC3410.8389509422821434No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGT2110.5191162722039069No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2010.49451360527481175No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGTGTGTT1520.3739605373222457No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1290.31737440338532696No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC1080.2657088028342272No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.25832800275549866No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1000.24602666929095116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG950.23372533582640356No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.20420213551148944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA820.20174186881857992No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGTGGGTA800.19682133543276092No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG740.18205973527530384No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG700.1722186685036658No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC690.16975840181075627No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC690.16975840181075627No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC670.16483786842493728No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.15991733503911823No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC620.1525365349603897No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC610.1500762682674802No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTG580.14269546818875165No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC570.14023520149584215No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT570.14023520149584215No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG560.13777493480293265No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT560.13777493480293265No Hit
TATATATACTATGTATTTAAGTACATAATATTAATAATGATTTAATACAT560.13777493480293265No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTG540.1328544014171136No Hit
GTATATATATATACTATGTATTTAAGTACATAATATTAATAATGATTTAA530.1303941347242041No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC520.12793386803129458No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGTGTG500.12301333464547558No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTG490.12055306795256605No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA490.12055306795256605No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA480.11809280125965654No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC460.11317226787383754No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTGCTAGTGGGTT450.11071200118092801No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTGTG440.1082517344880185No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG420.10333120110219947No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG420.10333120110219947No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC420.10333120110219947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)