FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18451
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTGTA4142.24378082488754No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC2301.2465449027153No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.9592975990461222No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATG1180.6395317326974148No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.4877784401929435No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA820.4444203566202374No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC760.4119017939407078No Hit
GCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGT580.31434610590211914No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG550.29808682456235436No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG490.2655682618828248No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG470.25472874098964826No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.24930898054305997No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC400.21679041786353045No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT390.21137065741694216No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.2059508969703539No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.2059508969703539No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA370.20053113652376567No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTG360.19511137607717738No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGGGTA350.18969161563058912No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA350.18969161563058912No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT330.1788520947374126No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC320.17343233429082436No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT290.15717305295105957No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC290.15717305295105957No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT290.15717305295105957No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC280.15175329250447128No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC280.15175329250447128No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC270.14633353205788305No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT260.14091377161129479No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG250.13549401116470652No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC250.13549401116470652No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC250.13549401116470652No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTGTAGA240.13007425071811826No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC220.11923472982494174No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.11381496937835348No Hit
CAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCA210.11381496937835348No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.10839520893176523No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTGTT190.10297544848517695No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10297544848517695No Hit
CAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCA190.10297544848517695No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA190.10297544848517695No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTTATGGTGTA190.10297544848517695No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10297544848517695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)