FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10352
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCT1751.6904945904173105No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCG970.9370170015455951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.8887171561051005No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGG620.5989180834621329No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG510.4926584234930448No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.40571870170015456No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT370.35741885625965997No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC320.3091190108191654No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA260.2511591962905719No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT250.24149922720247294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT240.23183925811437403No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT230.22217928902627512No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA220.2125193199381762No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC210.20285935085007728No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG210.20285935085007728No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTAT200.19319938176197837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT200.19319938176197837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT200.19319938176197837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC190.18353941267387944No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG170.16421947449768162No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG170.16421947449768162No Hit
CGTCAGATGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAA170.16421947449768162No Hit
ATTGCGCAATGACGACACGGGGGTTGTAGGACTAAAAAGAGGGTTACGAG150.14489953632148378No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT150.14489953632148378No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.14489953632148378No Hit
ATTGCGCAATGCTGGTACTGGGGTAGATAGGGTTTAAGAAGTGGTAGTTT140.13523956723338484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC140.13523956723338484No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT130.12557959814528594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.12557959814528594No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG120.11591962905718702No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC120.11591962905718702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT120.11591962905718702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT110.1062596599690881No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA110.1062596599690881No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT110.1062596599690881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)