FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10352
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTGTA1991.9223338485316845No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC1391.3427357032457496No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT730.705177743431221No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA720.6955177743431221No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATG560.5409582689335394No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.45401854714064915No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC460.44435857805255025No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.40571870170015456No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.38639876352395675No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC280.2704791344667697No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.2608191653786708No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT250.24149922720247294No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.24149922720247294No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.23183925811437403No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC230.22217928902627512No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT230.22217928902627512No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG230.22217928902627512No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC220.2125193199381762No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGGGTA200.19319938176197837No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.17387944358578053No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC170.16421947449768162No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG160.1545595054095827No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.14489953632148378No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTAATGGTG150.14489953632148378No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC150.14489953632148378No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGT140.13523956723338484No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT140.13523956723338484No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG130.12557959814528594No Hit
GTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11591962905718702No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC120.11591962905718702No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA110.1062596599690881No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA110.1062596599690881No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACGCGTTTATGGTGTA110.1062596599690881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)