FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21020
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCT3271.555661274976213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2521.1988582302568982No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCG1660.7897240723120837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1640.780209324452902No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAA1240.5899143672692674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA980.4662226450999048No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.4329210275927688No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT800.3805899143672693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT620.2949571836346337No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA610.29019980970504283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG610.29019980970504283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT600.285442435775452No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATAT580.2759276879162702No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT540.25689819219790677No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC530.25214081826831586No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC500.23786869647954328No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC490.2331113225499524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT440.20932445290199808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC430.20456707897240725No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG370.17602283539486205No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA360.17126546146527116No Hit
ATTGCGCAATGTATAACGTGGGGCGAACTCGGGGAACTGAAACATCTAAG360.17126546146527116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG360.17126546146527116No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC350.1665080875356803No Hit
ATTGCGCAATGCGCTACTCGGGGATCAAGCGACTAAGTGCATATGGTGGA340.16175071360608945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.16175071360608945No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG330.1569933396764986No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT320.1522359657469077No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG310.14747859181731685No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT310.14747859181731685No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG310.14747859181731685No Hit
ATTGCGCAATGCTCAACTAGGGCTTCCCGCGGTTAGACGGAAAGACCCCA300.142721217887726No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC300.142721217887726No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1379638439581351No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA270.12844909609895339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG270.12844909609895339No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT260.12369172216936251No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC250.11893434823977164No Hit
ATTGCGCAATGAGAGCCTTGGGGGATCAAGCGACTAAGTGCATATGGTGG250.11893434823977164No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCTCG240.11417697431018078No Hit
ATTGCGCAATGACTCCAGAGGGGGGTTGGAGTGTATAAAAAAAGGATAAT240.11417697431018078No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT240.11417697431018078No Hit
ATTGCGCAATGCTAGCATAGGGAGCGAACCTGGGGAACTGAAACATCTAA240.11417697431018078No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT240.11417697431018078No Hit
ATTGCGCAATGGGCAACGCGGGGGAAAGAGAAGAAGGATATATTAATTAA230.10941960038058991No Hit
AATCGAGCCCACGCCTCTGCGGAGCTACTTCCATACTCTGTGGCCAAGGG230.10941960038058991No Hit
ATTGCGCAATGAGAGTAAAGGGGGAGGGTGGTAGAAAAAGGAAAAAATAA220.10466222645099904No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGAT220.10466222645099904No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC220.10466222645099904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)