FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18244
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTGTA7143.9136154352115766No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC2351.288094716071037No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2151.1784696338522254No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.7125630344222758No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACT1130.6193817145362859No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA830.45494409120806834No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.44946283709712787No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC760.4165753124314843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC620.33983775487831613No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTG560.30695023021267265No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG560.30695023021267265No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT500.27406270554702916No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTGTAGA470.2576189432142074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA420.23021267265950449No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.22473141854856393No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.22473141854856393No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG400.21925016443762332No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG380.20828765621574216No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA370.20280640210480158No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC370.20280640210480158No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC330.18088138566103926No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA330.18088138566103926No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC310.16991887743915807No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.16991887743915807No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC300.16443762332821749No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA290.1589563692172769No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC290.1589563692172769No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.14251260688445516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.13703135277351458No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG250.13703135277351458No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA250.13703135277351458No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA240.131550098662574No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT240.131550098662574No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC230.12606884455163342No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC230.12606884455163342No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA220.12058759044069284No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.12058759044069284No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCC210.11510633632975224No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA210.11510633632975224No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCC200.10962508221881166No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10962508221881166No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC200.10962508221881166No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA190.10414382810787108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)