FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15112
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTGTA4943.268925357331922No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC2171.4359449444150345No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1711.1315510852302806No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.7146638433033351No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACT780.5161461090524087No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.496294335627316No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA730.48305982001058767No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC650.4301217575436739No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTG440.2911593435680254No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG430.28454208575966117No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA410.2713075701429328No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.2646903123345686No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC390.2580730545262043No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC380.25145579671784013No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC350.23160402329274749No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG340.2249867654843833No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT340.2249867654843833No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.2249867654843833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.2249867654843833No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG320.21175224986765487No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG280.18528321863419797No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAACTGTGTAGA270.17866596082583378No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC270.17866596082583378No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA260.17204870301746955No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC260.17204870301746955No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC250.16543144520910535No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCA250.16543144520910535No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT250.16543144520910535No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC240.15881418740074113No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC230.1521969295923769No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.13896241397564849No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC210.13896241397564849No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC200.1323451561672843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC200.1323451561672843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC200.1323451561672843No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.12572789835892006No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.11911064055055585No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA180.11911064055055585No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.11911064055055585No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.11249338274219164No Hit
TTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATGCCACAA160.10587612493382743No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCA160.10587612493382743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)