FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11820
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGT1921.6243654822335025No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGG1601.353637901861252No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC1361.1505922165820643No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.8375634517766498No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGA950.8037225042301184No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTT800.676818950930626No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.6345177664974619No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTA690.583756345177665No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTG520.4399323181049069No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.4230118443316413No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG440.3722504230118443No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA410.34686971235194586No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGA400.338409475465313No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG400.338409475465313No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG370.3130287648054145No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC360.3045685279187817No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC350.2961082910321489No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC340.2876480541455161No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC340.2876480541455161No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA330.27918781725888325No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC290.24534686971235195No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG270.2284263959390863No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG270.2284263959390863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC240.20304568527918782No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.19458544839255498No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG210.17766497461928935No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.17766497461928935No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG210.17766497461928935No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT210.17766497461928935No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA210.17766497461928935No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.1692047377326565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG200.1692047377326565No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC200.1692047377326565No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTG190.1607445008460237No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT190.1607445008460237No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG190.1607445008460237No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC180.15228426395939085No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.14382402707275804No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.14382402707275804No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC170.14382402707275804No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA160.1353637901861252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC160.1353637901861252No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAA160.1353637901861252No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAT150.12690355329949238No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG150.12690355329949238No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC150.12690355329949238No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA150.12690355329949238No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGG150.12690355329949238No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC140.11844331641285956No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA140.11844331641285956No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA130.10998307952622673No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCC130.10998307952622673No Hit
TTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAG120.10152284263959391No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA120.10152284263959391No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAA120.10152284263959391No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGA120.10152284263959391No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA120.10152284263959391No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCA120.10152284263959391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)