FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCAAATCT3582.59702575262967No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCAAATCG1751.2694958287994198No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.7544432354007979No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCA960.6964091403699674No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG870.6311207834602829No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT570.41349292709466806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA570.41349292709466806No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC540.39173014145810664No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.36996735582154516No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG490.35545883206383755No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT470.3409503083061299No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA450.3264417845484222No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCAAAT430.31193326079071454No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA380.2756619513964454No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA360.2611534276387377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT350.25389916575988397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT350.25389916575988397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT350.25389916575988397No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC340.2466449038810301No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.2466449038810301No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC320.23213638012332247No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC280.20311933260790715No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA270.19586507072905332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG260.18861080885019949No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG240.17410228509249184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT240.17410228509249184No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT230.166848023213638No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA230.166848023213638No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCAAATCTCG230.166848023213638No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC220.1595937613347842No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGA210.15233949945593037No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT210.15233949945593037No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA210.15233949945593037No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA190.1378309756982227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC190.1378309756982227No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.1378309756982227No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG190.1378309756982227No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC170.12332245194051505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT170.12332245194051505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC170.12332245194051505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT170.12332245194051505No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT160.11606819006166123No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.1088139281828074No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTG140.10155966630395358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.10155966630395358No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAATCCAAATCGCG140.10155966630395358No Hit
CGTCAGATGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAA140.10155966630395358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)