FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGT2351.7047515415306493No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGG1401.0155966630395359No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC1310.9503083061298512No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.7036634022488212No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGA910.6601378309756982No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.6166122597025753No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC600.4352557127312296No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA510.36996735582154516No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG510.36996735582154516No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTT490.35545883206383755No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA480.3482045701849837No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG460.333696046427276No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGGA450.3264417845484222No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTA450.3264417845484222No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTG450.3264417845484222No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC420.30467899891186073No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC420.30467899891186073No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG410.29742473703300687No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG350.25389916575988397No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG340.2466449038810301No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.22488211824446863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC290.21037359448676096No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG280.20311933260790715No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.19586507072905332No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC270.19586507072905332No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC270.19586507072905332No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG260.18861080885019949No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC260.18861080885019949No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT260.18861080885019949No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC260.18861080885019949No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG220.1595937613347842No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAG220.1595937613347842No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG220.1595937613347842No Hit
ACGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC210.15233949945593037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC210.15233949945593037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA210.15233949945593037No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC200.14508523757707653No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC190.1378309756982227No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG190.1378309756982227No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC180.13057671381936886No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA180.13057671381936886No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA180.13057671381936886No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGAAGGAGTGTG170.12332245194051505No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT170.12332245194051505No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC170.12332245194051505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA170.12332245194051505No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC170.12332245194051505No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCC160.11606819006166123No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC160.11606819006166123No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1088139281828074No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAAT140.10155966630395358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA140.10155966630395358No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA140.10155966630395358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC140.10155966630395358No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.10155966630395358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)