FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22132
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAGGACAAATCT4452.010663292969456No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAGGACAAATCG1840.8313753840592807No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG990.44731610337972166No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAGGACA960.4337610699439725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.37050424724380987No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC650.2936923911078981No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA630.284655702150732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT490.2213988794505693No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.2213988794505693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG480.21688053497198626No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT460.20784384601482017No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAGGACAAAT430.19428881257907105No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT420.18977046810048798No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT410.18525212362190493No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG400.1807337791433219No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC390.17621543466473885No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA390.17621543466473885No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA370.16717874570757274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT340.1536237122718236No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACA340.1536237122718236No Hit
ATTGCGCAATGCCGGGTGTGGGTACAGGGATGGGTGGAGTATTGTGGTCA330.14910536779324055No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT330.14910536779324055No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA300.1355503343574914No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC290.13103198987890835No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG290.13103198987890835No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACAGGACAAATCTCG270.12199530092174228No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11747695644315921No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT230.10392192300741009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)