FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22862
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCACTGATCT6572.8737643250809204No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCACTGATCG2731.1941212492345377No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.8879363135333742No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCACT1910.8354474674131748No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.7392179161928091No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1570.6867290700726096No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1270.5555069547721109No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1050.4592774035517453No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT940.41116262794156244No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA880.38491820488146267No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC760.3324293587612632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT700.3061849357011635No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCACTGAT690.30181086519114686No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCT640.2799405126410638No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC550.24057387805091418No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT540.23619980754089756No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC490.21432945499081446No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCACTGATCTCG490.21432945499081446No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCT480.20995538448079784No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG480.20995538448079784No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCT470.20558131397078122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC470.20558131397078122No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG460.2012072434607646No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT450.19683317295074798No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG440.19245910244073133No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG430.1880850319307147No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCT410.17933689091068147No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.161840608870615No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT360.15746653836059837No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT350.15309246785058175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC350.15309246785058175No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC350.15309246785058175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC340.14871839734056513No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA330.1443443268305485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT330.1443443268305485No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT320.1399702563205319No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA310.13559618581051527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC310.13559618581051527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG300.13122211530049865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT300.13122211530049865No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA300.13122211530049865No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT290.12684804479048203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG280.1224739742804654No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCT280.1224739742804654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT270.11809990377044878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT260.11372583326043216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGC260.11372583326043216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG260.11372583326043216No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT260.11372583326043216No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCAC260.11372583326043216No Hit
ATTGCGCAATGAAAAAAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.10935176275041554No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGC240.10497769224039892No Hit
ATTGCGCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCTCCA230.1006036217303823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)