FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22862
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGGG5302.3182573703088094No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGGT2240.9797917942437232No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.8529437494532413No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCAT1840.8048289738430584No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC1630.7129734931327093No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.46802554457177853No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA760.3324293587612632No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG720.31493307672119675No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.3061849357011635No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGG640.2799405126410638No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG630.27556644213104714No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC620.27119237162103055No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.25807016009098066No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGTG520.22745166652086432No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC510.2230775960108477No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC490.21432945499081446No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC490.21432945499081446No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.2012072434607646No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA450.19683317295074798No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC450.19683317295074798No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC440.19245910244073133No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC410.17933689091068147No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC400.17496282040066485No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG400.17496282040066485No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT390.17058874989064823No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGTT380.1662146793806316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC380.1662146793806316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC380.1662146793806316No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG350.15309246785058175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG340.14871839734056513No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT340.14871839734056513No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA310.13559618581051527No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC310.13559618581051527No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC300.13122211530049865No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAAC300.13122211530049865No Hit
ACGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC300.13122211530049865No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGGGGG280.1224739742804654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC280.1224739742804654No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC270.11809990377044878No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCA270.11809990377044878No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA260.11372583326043216No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA260.11372583326043216No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGTGTT260.11372583326043216No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA240.10497769224039892No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC240.10497769224039892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA240.10497769224039892No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.10497769224039892No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.1006036217303823No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA230.1006036217303823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)