FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31455
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCT6051.923382610077889No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3161.004609759974567No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCG3120.9918931807343825No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG2670.848831664282308No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1870.5945000794786203No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1750.5563503417580671No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCG1560.49594659036719124No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.4768717215069146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1180.3751390875854395No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA960.30519790176442535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG960.30519790176442535No Hit
ATTGCGCAATGAGAAGGCGGGGACCGACAATTCAAAAGAAAGCTAACCTT950.3020187569543793No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT910.28930217771419486No Hit
ATTGCGCAATGGCGTACATGGGGCCTATATTCTCTCCGTGTAAAAGGTTA880.2797647432840566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT880.2797647432840566No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC770.2447941503735495No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC770.2447941503735495No Hit
ATTGCGCAATGTGCATCCTGGGGGAGCATTCTATCTCAAAAGCTCCCTGG730.23207757113336513No Hit
ATTGCGCAATGCGAGTATTGGGCACATATGGCTATGGTTACAGCAGACGA700.22254013670322684No Hit
ATTGCGCAATGGATCCGATGGGGCTTTGGGTTCGAAAACATTTGCTGATC680.21618184708313465No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT680.21618184708313465No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT670.21300270227308854No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGAT650.20664441265299632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG650.20664441265299632No Hit
ATTGCGCAATGACAGACAGGGGGGACACAACAGAGACGATGGGGACGACG630.20028612303290413No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG620.19710697822285808No Hit
ATTGCGCAATGTGTAAGTTGGGGGTGTGGGGGACATTCCACGTTTTCCGC600.19074868860276584No Hit
ATTGCGCAATGTCAGATAAGGGGACCAGAACGTCGTCGTAACACCTCCTA600.19074868860276584No Hit
ATTGCGCAATGAGATTAGGGGGACACACACTCTTCACTTTCCCATCTTTG560.17803210936258146No Hit
ATTGCGCAATGAGTCGCACGGGGGACACAACAGAGACGATGGGGACGACG560.17803210936258146No Hit
ATTGCGCAATGGCTGCAGGGGGTCAAGTCGTCATTTACGATGCCAACAAC560.17803210936258146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA520.16531553012239708No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG520.16531553012239708No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA490.15577809569225878No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT480.15259895088221268No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG470.1494198060721666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG470.1494198060721666No Hit
ATTGCGCAATGTCATCTGGGGGGACCAAGCTCTTTCACTCTTCTCAAGAC460.14624066126212049No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC450.14306151645207438No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC440.1398823716420283No Hit
ATTGCGCAATGTTGGAAAAGGGGATTAGATACCCTGGTAGTCCATGCCGT440.1398823716420283No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCTCG430.1367032268319822No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG430.1367032268319822No Hit
ATTGCGCAATGCTGTCAAAGGGGATCACCGCATTCATCTCGACAATACTG430.1367032268319822No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1367032268319822No Hit
ATTGCGCAATGTATGCAACGGGAAAATCATATCCACTCCTTTGGTTCAGC430.1367032268319822No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT420.1335240820219361No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC420.1335240820219361No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT410.13034493721189No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG400.12716579240184392No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC380.1208075027817517No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA370.11762835797170561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT370.11762835797170561No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC370.11762835797170561No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA350.11127006835161342No Hit
ATTGCGCAATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGC350.11127006835161342No Hit
ATTGCGCAATGCAGATCGTGGGACCATTCCTTTCTACCACGCTGACGAGG350.11127006835161342No Hit
ATTGCGCAATGCTCCAAAGGGGGAAGTGACAATCATCTGAATCACATCAG340.10809092354156732No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT340.10809092354156732No Hit
ATTGCGCAATGGCGTGTTGGGGCTCTTGTTGAAGTTGGGTTGGGTAACAA340.10809092354156732No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCGCG330.10491177873152122No Hit
ATTGCGCAATGTCTATCCGGGGGATTCATCACTACAACACATCCTACCAC320.10173263392147512No Hit
ATTGCGCAATGATACTAAAGGGAACCTCTGGATTCTCTATCACCTTCGCA320.10173263392147512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)