FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17585
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCT4272.4282058572647145No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCG2061.171452942849019No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.8757463747512083No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1270.7222064259311913No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGG1180.6710264429911856No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.5288598237133921No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT860.4890531703156099No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA850.48336650554449817No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTAT660.37531987489337504No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT580.3298265567244811No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC570.32413989195336934No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA560.3184532271822576No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC430.24452658515780495No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT410.23315325561558145No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT390.22177992607335795No Hit
ATTGCGCAATGGTCAAGCATGGCGGACGACCTCGACACGGCCCAGCTCAA360.20471993176002273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG350.199033266988911No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT350.199033266988911No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGTAACGGTATCTCG330.18765993744668752No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT320.18197327267557578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.18197327267557578No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC310.17628660790446402No Hit
ATTGCGCAATGGTCCTCGGGGGCTACATAAATATATAAAAATAAAATAAA310.17628660790446402No Hit
ATTGCGCAATGGTCAGGGAGGGGAAAATGGCACCACACGCAGACTCCGAA310.17628660790446402No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG310.17628660790446402No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA300.17059994313335228No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.16491327836224054No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC290.16491327836224054No Hit
ATTGCGCAATGCTGGGGCAGGGAGCGAACCTGGGGAACTGAAACATCTAA280.1592266135911288No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT280.1592266135911288No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC280.1592266135911288No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA270.15353994882001706No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT270.15353994882001706No Hit
ATTGCGCAATGGAAATGGAGGGGGAACAGGATGAACATTATATCCACCTT260.14785328404890533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG260.14785328404890533No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT250.1421666192777936No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGT250.1421666192777936No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC240.13647995450668185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT240.13647995450668185No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG230.13079328973557008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT230.13079328973557008No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA220.12510662496445835No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA220.12510662496445835No Hit
ATTGCGCAATGAGCCGGGTGGGGCAGCTCGAGTTAAGTTTAACAAACATA210.1194199601933466No Hit
ATTGCGCAATGTGTGATCAGGGAGCCCCAAGGTTTCGCCGTCAACGGCCA210.1194199601933466No Hit
ATTGCGCAATGTAATCTCCGGGGGAACGAACTCCAGCCGACAAGATGGGA210.1194199601933466No Hit
ATTGCGCAATGCGTACCAAGGGGAACGCCAACCTACCGCACAAACTTCCA210.1194199601933466No Hit
ATTGCGCAATGAAAAAAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1194199601933466No Hit
ATTGCGCAATGGAGTTAGCGGGGAAGGTCTTAAGTCGTCTTTCCAAGTCC210.1194199601933466No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC200.11373329542223486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT200.11373329542223486No Hit
ATTGCGCAATGAACTGGGGGGGGTATCACTTGGACGTTCGAGGTTATAGC200.11373329542223486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG200.11373329542223486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC190.1080466306511231No Hit
ATTGCGCAATGCACCACAAGGGCTTATGGACAATTCTGTTTTTCAAGTAT190.1080466306511231No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT180.10235996588001137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)