FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28408
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCT5481.9290340749084767No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCG2550.8976344691636159No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.8448324415657561No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1780.6265840608279358No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAA1640.5773021684032666No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.5491410870177414No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1130.3977752745705435No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1060.3731343283582089No Hit
ATTGCGCAATGGCTCTAGTGGGGATTCACAGAGAATATTCAATAAACACA1050.3696141931850183No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT910.32033230076034924No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC910.32033230076034924No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA760.26753027316248945No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG680.23936919177696422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT670.2358490566037736No Hit
ATTGCGCAATGCGATCAACGGGAAGACCAGGGTCACAGAGCGGGCCAACA660.23232892143058292No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATAT610.21472824556462966No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT600.21120811039143902No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT580.20416784004505772No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG560.19712756969867645No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC550.1936074345254858No Hit
ATTGCGCAATGGATGCATGGGGCTTCGTGATAGCTGGTTCTCTCCGAAAT530.18656716417910446No Hit
ATTGCGCAATGGGAGACAAGGGGAGACACGTCCGCTTTCTGGGAGCTACC530.18656716417910446No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG510.17952689383272316No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT490.17248662348634186No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG480.16896648831315123No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCTCG470.16544635313996056No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG470.16544635313996056No Hit
ATTGCGCAATGTCCTGACTGGGGTAGATATTCGGAGGAACACCAGTGGCG430.151365812447198No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG430.151365812447198No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG430.151365812447198No Hit
ATTGCGCAATGAACTGGCCGGGGAAGGTCAATTCCTTGAAAGCACCGGTC420.14784567727400733No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC420.14784567727400733No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC410.14432554210081666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC400.14080540692762603No Hit
ATTGCGCAATGGGTCGGGTGGGTTGAGAGTTTGATCCTGGCTCAGAGCGA370.13024500140805406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA370.13024500140805406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC370.13024500140805406No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC350.12320473106167276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT350.12320473106167276No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT350.12320473106167276No Hit
ATTGCGCAATGCTATACGAGGGGATGCACTATAAGCTGACTATACAGGGA340.11968459588848211No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG340.11968459588848211No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT340.11968459588848211No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC330.11616446071529146No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG320.11264432554210081No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG320.11264432554210081No Hit
ATTGCGCAATGACAGCTTTGGGCTAGACAAGGACATCTCGCTTGGCTTTA310.10912419036891016No Hit
ATTGCGCAATGCGGTACTCGGGGGTATCAACGGGTAACGGGGAATTAGGG310.10912419036891016No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG300.10560405519571951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT290.10208392002252886No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG290.10208392002252886No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT290.10208392002252886No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA290.10208392002252886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)