FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22976
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTGGTGCTAATCT4712.0499651810584956No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTGGTGCTAATCG2040.8878830083565459No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000.8704735376044569No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1730.7529596100278552No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.6093314763231198No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTGGTGCT1200.5222841225626741No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1070.4657033426183844No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA850.36995125348189417No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT840.36559888579387184No Hit
GAATTAATACTGCTGCCGATGTTGGGATCCATCCAAAAAGTACTTTCATT730.3177228412256268No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC700.3046657381615599No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA660.28725626740947074No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTGGTGCTAAT580.2524373259052925No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT570.24808495821727017No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT540.23502785515320335No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG530.23067548746518104No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT520.22632311977715877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC480.20891364902506965No Hit
ATTGCGCAATGGCTAGGCGGGACGACTTGTCCATATACGTGAACTCGACA470.20456128133704735No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC460.20020891364902507No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTG460.20020891364902507No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT440.1915041782729805No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTG390.16974233983286907No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTGGTGCTAATCTCG360.15668523676880222No Hit
ATTGCGCAATGCGCATGATGGGGACAGATCGCCCACTACCTACGGTCTCC340.14798050139275765No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTG330.14362813370473537No Hit
ATTGCGCAATGTGCCGGGTGGGAGGAAACCGTGCCGGTACTAGACCTTTT330.14362813370473537No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG330.14362813370473537No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC320.1392757660167131No Hit
ATTGCGCAATGGAATAAAGGGGGAGGACTCTGGATTTGTGTCACTTTCTG310.1349233983286908No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT310.1349233983286908No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG310.1349233983286908No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGTG300.13057103064066852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT300.13057103064066852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA300.13057103064066852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT300.13057103064066852No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT300.13057103064066852No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC290.12621866295264625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC280.12186629526462396No Hit
ATTGCGCAATGTCAGAAGCGGGAGCGAACCTGGGGAACTGAAACATCTAA280.12186629526462396No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA270.11751392757660167No Hit
ATTGCGCAATGCTATCGCCGGGATGTTTCTGCAGACCCAGAACAGGTAAA270.11751392757660167No Hit
ATTGCGCAATGGGTGTACGGGGAGTCCACGCCCTAAACGATGTCAACTAG260.11316155988857939No Hit
ATTGCGCAATGCTCTAACTGGGGGAAAACGTTCCTGGTGTCTTATGCTTT260.11316155988857939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC260.11316155988857939No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT250.10880919220055711No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG250.10880919220055711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.10880919220055711No Hit
ATTGCGCAATGTCGACAGTGGGAAGCAACCAAGAAGGATTCTCATGGTTC250.10880919220055711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG250.10880919220055711No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC250.10880919220055711No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC240.10445682451253482No Hit
ATTGCGCAATGTAGTGACGGGCGGTGTGTACAAAGGGCAGGGACGAATTC240.10445682451253482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG240.10445682451253482No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG230.10010445682451254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGTC230.10010445682451254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT230.10010445682451254No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC230.10010445682451254No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)