FastQCFastQC Report
Wed 8 May 2024
EGAF00007816496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94749
Total Bases14.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGGG6530.6891893318135284No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC2770.2923513704630128No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCATGGGGT2770.2923513704630128No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCAACATCAT2460.25963334705379476No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.25224540628397135No Hit
ACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCC2030.21425028232487944No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.197363560565283No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA1830.1931418801253839No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG1760.18575393935556048No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG1330.14037087462664516No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG1290.13614919418674604No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA1290.13614919418674604No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG1240.13087209363687213No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1160.12242873275707396No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1160.12242873275707396No Hit
CTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAAT1080.11398537187727575No Hit
GTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAAT1070.11292995176730097No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT1040.10976369143737665No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG1040.10976369143737665No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG1030.10870827132740186No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1020.10765285121742708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)