FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331011
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT8700.26283114458431894No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG8470.25588273501484843No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG7750.2341311920147669No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG7720.23322487772309683No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC7560.22839120150085646No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG7310.22083858240360593No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA7090.21419227759802545No Hit
CTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAAT6680.20180598227853455No Hit
GTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAAT6480.1957638870007341No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC6270.18941968695904365No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG6000.18126285833401307No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5980.18065864880623303No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA5800.17522076305621265No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT5740.1734081344728725No Hit
CCCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGT5530.16706393443118203No Hit
GATTAGGACGGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAG5490.16585551537562196No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT5410.16343867726450179No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5400.16313657250061178No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA5140.1552818486394712No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT5130.15497974387558117No Hit
GGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAG4900.14803133430611068No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC4710.14229134379220026No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4570.13806187709773995No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA4520.13655135327828985No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG4490.13564503898661978No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG4450.13443661993105968No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT4300.12990504847270937No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC4260.12869662941714927No Hit
AGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGCAGATG4240.12809241988936926No Hit
GTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGGACT4220.1274882103615892No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG4220.1274882103615892No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4190.12658189606991913No Hit
CGATAAACCCTAGGAAGCCAATTGATATCATAGCTCAGACCATACCTATG4140.12507137225046902No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG4110.12416505795879895No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG4100.12386295319490893No Hit
CGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACCCTAGG4080.12325874366712888No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT4080.12325874366712888No Hit
GAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCC3980.12023769602822866No Hit
GGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAG3950.1193313817365586No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG3870.11691454362543843No Hit
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA3850.1163103340976584No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.11510191504209831No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC3780.11419560075042823No Hit
CTCCTGATGCGAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGA3780.11419560075042823No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA3780.11419560075042823No Hit
AGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCTACG3760.11359139122264819No Hit
GGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTAC3740.11298718169486814No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG3700.11177876263930805No Hit
GGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATG3690.11147665787541804No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA3680.11117455311152802No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG3680.11117455311152802No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT3640.10996613405596792No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG3630.10966402929207791No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT3590.10845561023651781No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT3540.10694508641706771No Hit
TCATAGCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAG3540.10694508641706771No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT3510.10603877212539765No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT3500.10573666736150762No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG3480.10513245783372759No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT3410.10301772448649742No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG3360.10150720066704731No Hit
ATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTACAAT3360.10150720066704731No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT3350.1012050959031573No Hit
GAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGG3340.10090299113926728No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC3320.10029878161148724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)