FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27151
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC4091.506390188206696No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGG3761.3848477035836617No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGT2781.0239033553091967No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.9023608706861626No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAG1780.6555927958454568No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.6408603734669073No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1720.6334941622776324No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1560.5745644727634341No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.34621192589591543No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG870.32043018673345364No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.29833155316562926No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTG800.29464844757099184No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG750.2762329195978049No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC650.2394018636514309No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA650.2394018636514309No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG640.23571875805679351No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC620.2283525468675187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC620.2283525468675187No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.2283525468675187No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA550.20257080770505692No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA530.1952045965157821No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG520.1915214909211447No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC500.1841552797318699No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA490.18047217413723252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC490.18047217413723252No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC460.16942285735332033No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC450.1657397517586829No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTT440.16205664616404553No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC430.1583735405694081No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA410.15100732938013334No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCC390.14364111819085854No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA370.13627490700158373No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAAAAT370.13627490700158373No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT360.13259180140694635No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG350.12890869581230893No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG340.12522559021767155No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT330.12154248462303413No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGA320.11785937902839676No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTA320.11785937902839676No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG320.11785937902839676No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC310.11417627343375936No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCC300.11049316783912194No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC300.11049316783912194No Hit
CCCCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTT300.11049316783912194No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA290.10681006224448454No Hit
CGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10312695664984714No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA280.10312695664984714No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT280.10312695664984714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)