FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15406
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC3552.3042970271322862No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGG2781.8044917564585226No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGT1721.1164481370894457No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1400.908736855770479No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1360.8827729456056082No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.8113721926522134No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.6815526418278592No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAG1050.6815526418278592No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.3505127872257562No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC520.3375308321433208No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTG520.3375308321433208No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG480.31156692197844993No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA470.30507594443723224No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.30507594443723224No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG400.2596391016487083No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.22718421394261976No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.22069323640140204No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC330.21420225886018435No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.20771128131896666No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT300.19472932623653122No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG290.18823834869531353No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC290.18823834869531353No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC290.18823834869531353No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA280.1817473711540958No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG280.1817473711540958No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTA270.1752563936128781No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC250.16227443853044268No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC250.16227443853044268No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC250.16227443853044268No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTT240.15578346098922496No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC230.14929248344800727No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.14929248344800727No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA230.14929248344800727No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT230.14929248344800727No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA220.14280150590678956No Hit
CAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCA220.14280150590678956No Hit
ACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.13631052836557186No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA210.13631052836557186No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG200.12981955082435415No Hit
CAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCA200.12981955082435415No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAAAA200.12981955082435415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.12981955082435415No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCC190.12332857328313644No Hit
GTGTAGGAGATACAAGTGACCACCTGGAGTTTCTGTACTTCATTCTGCTC180.11683759574191872No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGATTTTTTTT180.11683759574191872No Hit
CATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCA180.11683759574191872No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.11683759574191872No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.11034661820070102No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170.11034661820070102No Hit
CCCCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTT170.11034661820070102No Hit
CGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.10385564065948333No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT160.10385564065948333No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC160.10385564065948333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)