FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCT5041.7722142128766833No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3261.146313161503569No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG2550.8966560005626076No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCG2540.8931397025211858No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAA2160.75952037694715No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.74193888674004No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1620.5696402827103625No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1570.5520587925032526No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC1050.36921129434930905No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.3621786982664651No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG970.3410809100179331No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT830.29185273743802526No Hit
ATTGCGCAATGATTCACAGGGGAGGTATATACCTTTTTGCTTGGCTGGGG780.2742712472309153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT780.2742712472309153No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC740.2602060550652273No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG720.25317345898238336No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT710.24965716094096135No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC700.24614086289953935No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG650.2285593726924294No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT630.22152677660958542No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATAT610.21449418052674143No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT600.21097788248531946No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG600.21097788248531946No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA570.2004289883610535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC570.2004289883610535No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG550.1933963922782095No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG540.1898800942367875No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC530.18636379619536553No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG520.18284749815394352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCT510.17933120011252154No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG500.17581490207109954No Hit
ATTGCGCAATGGTACTTTCGGGATGGCATCCCACTCGCACAGGACAATCG480.16878230598825558No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA480.16878230598825558No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG470.16526600794683358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC460.1617497099054116No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.15471711382256761No Hit
ATTGCGCAATGAGCACTACGGGGATCGGGCGCCGGGACCGCGCATGGCAG420.14768451773972363No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC410.14416821969830163No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC400.14065192165687962No Hit
ATTGCGCAATGAAGGACACGGGCAACCATCTCAAGTGCAGCCTTGCAGCT400.14065192165687962No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT390.13713562361545764No Hit
ATTGCGCAATGGATATACCGGGATAGTGAACCAGTACCGTGAGGGAAAGG390.13713562361545764No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT380.13361932557403564No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT370.13010302753261366No Hit
ATTGCGCAATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGG370.13010302753261366No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGGAGCAATATCTCG360.12658672949119168No Hit
ATTGCGCAATGATGCTCGTGCTGTCTCTTATACACATCTCCGAGCCCACG360.12658672949119168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTGA350.12307043144976967No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT350.12307043144976967No Hit
ATTGCGCAATGAAAAAAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11955413340834768No Hit
ATTGCGCAATGCGGAACACGGGAAGGCGCCTGCAGGAGTGGATGACTGTC330.11603783536692569No Hit
ATTGCGCAATGGAGAATCAGGGTCAAACCTGAGAGTTTGATCCTGGCTCA330.11603783536692569No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.10900523928408172No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGTC300.10548894124265973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTCT300.10548894124265973No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG290.10197264320123774No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA290.10197264320123774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)