FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007816532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28439
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC4801.6878230598825557No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGG3791.3326769576989346No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.9634656633496256No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGT2480.8720419142726538No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAG2140.752487780864306No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.6997433102429762No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA1870.6575477337459122No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC1720.6048032631245824No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.41843946692921696No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG970.3410809100179331No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC780.2742712472309153No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.24262456485811737No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG660.23207567073385138No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.2285593726924294No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG630.22152677660958542No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA630.22152677660958542No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTG600.21097788248531946No Hit
TCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA600.21097788248531946No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACC600.21097788248531946No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG590.20746158444389745No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCC580.20394528640247547No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT580.20394528640247547No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC570.2004289883610535No Hit
GCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA530.18636379619536553No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC520.18284749815394352No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA500.17581490207109954No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC480.16878230598825558No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG480.16878230598825558No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC470.16526600794683358No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC460.1617497099054116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC460.1617497099054116No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC440.15471711382256761No Hit
CGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACG420.14768451773972363No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA360.12658672949119168No Hit
GCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.12658672949119168No Hit
CACGAGCATCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGA350.12307043144976967No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT350.12307043144976967No Hit
CAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCA340.11955413340834768No Hit
CCACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTT340.11955413340834768No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA330.11603783536692569No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAATTTA330.11603783536692569No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA330.11603783536692569No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTG330.11603783536692569No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA330.11603783536692569No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT320.11252153732550371No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTT320.11252153732550371No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGTA320.11252153732550371No Hit
CACGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTT320.11252153732550371No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA310.10900523928408172No Hit
CCATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATAAGA310.10900523928408172No Hit
CAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCA300.10548894124265973No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.10548894124265973No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATAAGAGTGAGGTGGGGG300.10548894124265973No Hit
CGAGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.10197264320123774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)