FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007816579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007816579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14379
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.9666875304263162No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCT1380.9597329438764864No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGATCG1050.7302315877321094No Hit
GAACTGCAGCGAGAGCACAAAGATTCTAGGATACTGCGAGCAAATGGGGT840.5841852701856874No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.5633215105361986No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA550.3825022602406287No Hit
ATTGCGCAATGTGTGCACTGGGCGGCAGGTCCATGGTCAGCTCTAGAATT520.3616385005911399No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG470.3268655678419918No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCG460.31991098129216217No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC330.22950135614437725No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA300.2086375964948884No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT250.17386466374574033No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGTGCGGAT250.17386466374574033No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1669100771959107No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC230.15995549064608108No Hit
ATTGCGCAATGAGTTAAGAGGGGAGACACGGCCCAGACTCCTACGGGAGG200.13909173099659225No Hit
ATTGCGCAATGGGTGGTGCGGGTCGCATGCGCCAAAACGAGATCGAAGAC200.13909173099659225No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.13909173099659225No Hit
ATTGCGCAATGTGGGGGGTGGGGCCATCGCGGCAGCAGGGAAGATGGTGA190.13213714444676264No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG180.12518255789693303No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT170.1182279713471034No Hit
CGTCAGATGTGTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAA170.1182279713471034No Hit
GTACCCTCTTGATTTTGCCCGTACCCGTCTAGCAGCTGATGTGGGTAAAG160.1112733847972738No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG160.1112733847972738No Hit
ATTGCGCAATGCAGACCTCGGGATAACGGTCCTAAGGTAGCGAAATTCCT160.1112733847972738No Hit
ATTGCGCAATGCGTACTCCGGGGTTGAGCCGCGCCAAGGAATCGAGAGCT150.1043187982474442No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG150.1043187982474442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA150.1043187982474442No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT150.1043187982474442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)