FastQCFastQC Report
Sun 2 Apr 2023
EGAF00007818599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007818599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29868727
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1682650.5633484145474296TruSeq Adapter, Index 3 (100% over 50bp)
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCC436270.1460624686147488No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN421670.14117441295707045No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC409130.13697604186479057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)