FastQCFastQC Report
Sat 1 Apr 2023
EGAF00007818631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007818631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28083395
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1366680.4866505634379319TruSeq Adapter, Index 7 (97% over 36bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1198110.42662576942709385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT785580.2797311364954273TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN674870.24030926460280175No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA562420.20026780950095244No Hit
CACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC496570.17681978977256846No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC453470.16147264246363374No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC427180.15211123868748774No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC327070.11646383921886938No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA297210.10583122161690209No Hit
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCG283970.10111669190993468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)