FastQCFastQC Report
Fri 31 Mar 2023
EGAF00007818634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007818634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18252145
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6883793.7714964460341514TruSeq Adapter, Index 19 (97% over 40bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC1328710.7279747120133004No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1238350.6784682019565371TruSeq Adapter, Index 19 (97% over 40bp)
CCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCG551790.3023151525478238No Hit
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA476540.26108712154105723No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG462340.2533072140288169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN428560.23479980024265643No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCC405930.22240125749603676No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC392710.21515827317830316No Hit
CGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCAC361290.1979438581054446No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG348730.19106247512278693No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA345370.18922159559876386No Hit
TGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC343940.1884381260394326No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA340990.18682187764780522No Hit
CTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCA334290.1831510762159735No Hit
CGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGA323930.1774750310169024No Hit
AGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGG323710.17735449723854374No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA286310.1568637549175727No Hit
AGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGC280280.15356003362892418No Hit
CTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCC265030.14520485126542662No Hit
CTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAG263880.14457478833309728No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG252400.13828511662601847No Hit
CCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGGC240600.1318201230595089No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAA219100.12004068562900415TruSeq Adapter, Index 19 (97% over 40bp)
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACG217710.11927913130210174No Hit
CAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC202990.11121432576828641No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196180.10748325744727538No Hit
CAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCA188430.10323718116418647No Hit
CTCCATGATGTGTTCTATGACCTTTTCATTCTTATTCTCCTTCTTGAGAA186490.10217429239138742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)