FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007820865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007820865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486512782
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG93969181.9314842996252461TruSeq Adapter, Index 27 (97% over 38bp)
TATTATGCTTCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT34756320.7143968521673908No Hit
TATTATGCTTCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA26204370.5386162701065478No Hit
TATTATGCTTCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC8444110.1735639907606785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)