FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007820921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007820921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504282736
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG72907211.4457605782483103TruSeq Adapter, Index 7 (97% over 34bp)
CCGTAAATTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT26509870.5256945778131893No Hit
CCGTAAATTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA17851610.35400002271741465No Hit
CCGTAAATTACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5400220.10708714803197229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)