FastQCFastQC Report
Sun 19 Mar 2023
EGAF00007820923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007820923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503877365
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG69975921.3887490262635631TruSeq Adapter, Index 7 (97% over 34bp)
CCGTAAATTACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT26697260.5298364612984748No Hit
CCGTAAATTACTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA18047530.35817306459082554No Hit
CCGTAAATTACGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5459960.10835890594132959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)