FastQCFastQC Report
Tue 7 May 2024
EGAF00007822053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9918
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG4014.043153861665658No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC3023.0449687436983264No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT2482.5005041338979637No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1341.3510788465416415No Hit
ATTGCGCAATGACGACTCAGGGGATGGCCGCCTCCGTGGATCTGGAGTTG1201.2099213551119177No Hit
ATTGCGCAATGTACAAGCCGGGGAGGGTACCTGCGCGGGACCAAGCGGGA950.9578544061302682No Hit
ATTGCGCAATGTTAAAAAGGGGCAGCTAGGAATAATGGAATAGGACCGCG910.9175236942932044No Hit
ATTGCGCAATGCCCAACTCGGGGGGGAGGTAGTGACGAAAAATAACAATA750.7562008469449486No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.7057874571486187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG630.6352087114337568No Hit
ATTGCGCAATGGAGGTCGCGGGGGAGGTCAGGAAAGGAGATTGAGAAGGA590.594877999596693No Hit
ATTGCGCAATGGAATGGCCGGGGCGAGCGCTCTTGCGCCGAAGATGAACG550.554547287759629No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAT540.5444646098003629No Hit
ATTGCGCAATGCGCCCAAGGGGGTAACACTGAATGGAGGACCGAACCGAC530.534381931841097No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.4638031861262351No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCG440.44363783020770314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT340.34281105061504336No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT330.33272837265577737No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT300.3024803387779794No Hit
ATTGCGCAATGTAAGACCGGGGCGAGCGCTCTTGCGCCGAAGATGAACGG290.29239766081871343No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA280.28231498285944745No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC280.28231498285944745No Hit
ATTGCGCAATGGATGCCCGGGGCGAGCGCTCTTGCGCCGAAGATGAACGG270.27223230490018147No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG250.2520669489816495No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG200.2016535591853196No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT190.19157088122605362No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA190.19157088122605362No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT170.17140552530752168No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC170.17140552530752168No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT160.1613228473482557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT160.1613228473482557No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT150.1512401693889897No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT140.14115749142972372No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTCT130.13107481347045777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC130.13107481347045777No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC130.13107481347045777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG130.13107481347045777No Hit
ATTGCGCAATGTTCATGGTGGGTAGATCATGAGGTCAGGAGTTCAAGACC120.12099213551119178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG120.12099213551119178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG120.12099213551119178No Hit
ATTGCGCAATGGTACGATTGGGCGGTCCGGAAGCTAAGAAGATCAAGACG120.12099213551119178No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG120.12099213551119178No Hit
ATTGCGCAATGTTTCGGGGGGGGGATCGTAAAACTCTGTTATTAGGGAAG110.11090945755192579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT110.11090945755192579No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.11090945755192579No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC110.11090945755192579No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCTCG110.11090945755192579No Hit
ATTGCGCAATGATCTCACAGGGGGGCGCGCGGGCGGCCTGATGTCGCCGC100.1008267795926598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG100.1008267795926598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT100.1008267795926598No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCGCG100.1008267795926598No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG100.1008267795926598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)