FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10860
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA7196.620626151012892No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT4744.364640883977901No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3122.8729281767955803No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG790.7274401473296501No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC620.570902394106814No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.5524861878453038No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC570.5248618784530387No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG470.43278084714548803No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA470.43278084714548803No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.4143646408839779No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC410.3775322283609577No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA400.3683241252302026No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC370.3406998158379374Illumina Single End PCR Primer 1 (100% over 22bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG330.30386740331491713No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG280.2578268876611418No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG250.23020257826887663No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC240.22099447513812157Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG240.22099447513812157No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG220.20257826887661143No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC220.20257826887661143No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA210.19337016574585636No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG210.19337016574585636No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT200.1841620626151013No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGC190.17495395948434622No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG190.17495395948434622No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC190.17495395948434622No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA180.16574585635359115No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCC180.16574585635359115No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA180.16574585635359115No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA170.15653775322283608No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.15653775322283608No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT170.15653775322283608No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG170.15653775322283608No Hit
GGATCATTTACCAAGGCTCTTGCAATAGCTACTCTCTGTCTTTGCCCTCC150.13812154696132595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTT150.13812154696132595No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGGA150.13812154696132595No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT150.13812154696132595No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA150.13812154696132595No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC140.1289134438305709Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA140.1289134438305709No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGGC120.11049723756906078No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC120.11049723756906078No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGGGCC120.11049723756906078No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT120.11049723756906078No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA120.11049723756906078No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATATCGG110.10128913443830571No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA110.10128913443830571No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGGC110.10128913443830571Illumina Single End PCR Primer 1 (95% over 22bp)
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.10128913443830571No Hit
GCAGCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGAC110.10128913443830571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)