FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14611
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC7365.037300663883375No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG4272.922455684073643No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC4182.860858257477243No Hit
ATTGCGCAATGGGATAGTGGGGGAGGGGAGTGAAACAGTACCTGAAACCG3182.1764424064061325No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT3182.1764424064061325No Hit
ATTGCGCAATGGTACTCCGGGGCAGACACCACAAAAGGTGTTAGTTCATC2241.5330915063992883No Hit
ATTGCGCAATGGGGGCCCGGGGGGGTCAGTGACTTAGTGTAACAAGCAAG1881.2867018000136883No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.7254808021353775No Hit
ATTGCGCAATGTGAAAGTGGGGAGCTACTAAAGACTGTTGGATTAAGGGT940.6433509000068441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC910.6228184244747108No Hit
ATTGCGCAATGGCGTCCCAGGGCTACTTGGATAACTGTGGTAATTCTAGA890.6091301074532887No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATA890.6091301074532887No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCG850.5817534734104443No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG750.5133118883033331No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATAT750.5133118883033331No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT620.42433782766408873No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC590.4038053521319554No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG570.3901170351105332No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT560.38327287659982207No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT500.3422079255355554No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACCGATATATCT440.30114297447128874No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACA440.30114297447128874No Hit
ATTGCGCAATGAGGGGAGTGGGGAAACCCAAGTTGGTCTTGATCACAAAC440.30114297447128874No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT420.28745465744986654No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC380.26007802340702213No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC360.2463897063855999No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG340.23270138936417767No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG340.23270138936417767No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC340.23270138936417767No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCG330.22585723085346657No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA320.21901307234275547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT310.21216891383204436No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG300.20532475532133326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG300.20532475532133326No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC290.1984805968106221No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT260.1779481212784888No Hit
ATTGCGCAATGGATTGTCGGGGGGAGCCCCGGCGTCTCCTGTGCAAAGGG250.1711039627677777No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT240.16425980425706657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.15741564574635547No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTG220.15057148723564437No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC220.15057148723564437No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.15057148723564437No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTG210.14372732872493327No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT200.13688317021422217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT200.13688317021422217No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT190.13003901170351106No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACGGACAGATATATCT180.12319485319279995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG180.12319485319279995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT180.12319485319279995No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT170.11635069468208883No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACAGAT160.10950653617137773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTC160.10950653617137773No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT150.10266237766066663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG150.10266237766066663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCAGTC150.10266237766066663No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGTG150.10266237766066663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)