FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14611
Sequences flagged as poor quality0
Sequence length151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT9316.371911573472041No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA8936.11183355006502No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC5193.552118267059065No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG1130.7733899117103552No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA910.6228184244747108No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG900.6159742659639997No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC720.4927794127711998No Hit
CCTTCAACCTGGTCATGCCTAGATCACCGGGTTTCGGGTCTAATGCAACA680.46540277872835534No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC670.4585586202176442No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG590.4038053521319554No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC550.37642871808911094Illumina Single End PCR Primer 1 (100% over 22bp)
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.36274040106768873No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA390.2669221819177332No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG370.253233864896311No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGC360.2463897063855999No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG360.2463897063855999No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG350.23954554787488877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC350.23954554787488877No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.23270138936417767No Hit
GGTTTCGGGTCTAATGCAACAAACTCAGTCGCCCTATTCAGACTCGCTTT330.22585723085346657No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG300.20532475532133326No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG290.1984805968106221No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCC290.1984805968106221No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC290.1984805968106221No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC280.19163643829991103Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC260.1779481212784888No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC260.1779481212784888No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT250.1711039627677777No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT240.16425980425706657No Hit
GGTATTCATCCGACGCTCTACCTCAATAGATTTCGCGGAGAACCAGCTAT240.16425980425706657No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC230.15741564574635547Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG230.15741564574635547No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.15741564574635547No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA230.15741564574635547No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGGA230.15741564574635547No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA200.13688317021422217No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGGGG190.13003901170351106No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.13003901170351106No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.12319485319279995No Hit
GTATTCATCCGACGCTCTACCTCAATAGATTTCGCGGAGAACCAGCTATT170.11635069468208883No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTC170.11635069468208883No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA170.11635069468208883No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT170.11635069468208883No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA160.10950653617137773No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAAAT150.10266237766066663No Hit
GGGTTTCGGGTCTAATGCAACAAACTCAGTCGCCCTATTCAGACTCGCTT150.10266237766066663No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)