FastQCFastQC Report
Tue 7 May 2024
EGAF00007822062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTA10857.173079465820442No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5263.4774560359645643No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3372.227951870950681No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGG1290.852836176120587No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTA830.5487240513023932No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTG800.5288906518577284No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.4958349861166204No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTC650.4297236546344043No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.3834457225968531No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCG570.3768345894486315No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC520.34377892370752344No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA500.33055665741108026No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTCGCC490.32394552426285866Illumina Single End PCR Primer 1 (100% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG440.29088985852175064No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG430.28427872537352905No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG420.2776675922253074No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC420.2776675922253074No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA340.22477852703953458No Hit
GGATTCCCACCCGTCACACGCTACTCATGCCTGCATTCTCACTCGCATCA330.21816739389131298No Hit
ATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGG330.21816739389131298No Hit
CTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGTTCTCGG320.2115562607430914No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC320.2115562607430914No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGTGGCC300.19833399444664812Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA300.19833399444664812No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGCGCC290.19172286129842656Illumina Single End PCR Primer 1 (95% over 22bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC270.17850059500198334No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG250.16527832870554013No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC250.16527832870554013No Hit
CATCTGACGCTGCCGACGATTCCGCATTAGTGTAGATCTCGGTGGGGGCC240.15866719555731854No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGTAGA240.15866719555731854No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.15205606240909691No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCA220.14544492926087532No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT200.1322226629644321No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATTAGTGGA200.1322226629644321No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCCGCATT200.1322226629644321No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG200.1322226629644321No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT180.1190003966679889No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAAT180.1190003966679889No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.1190003966679889No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.11238926351976729No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCAA170.11238926351976729No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA160.1057781303715457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)