FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15517
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG5403.4800541341754205No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5373.460720500096668No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT3652.3522588129148674No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3372.1718115615131794No Hit
ATTGCGCAATGTGGAGTGGGGGAGAGAGAGGTGAGTGAAATGAGATATGA2011.2953534832764064No Hit
ATTGCGCAATGAGCTCGGCGGGGGGGAAGTGAAACATCTCAGTACCCACA1921.2373525810401496No Hit
ATTGCGCAATGCGCGTGTAGGGGAGGTTCATCACAAATAACTAAATTATA1260.8120126313075982No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.7991235419217633No Hit
ATTGCGCAATGGCGCCATGGGGAAGTCGCAAATTGGAAAGGTCGATGAGA1030.6637881033704969No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCAC1030.6637881033704969No Hit
ATTGCGCAATGCGCATGGAGGGGAGGTTCATCACAAATAACTAAATTATA1030.6637881033704969No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG890.5735644776696527No Hit
ATTGCGCAATGAACCCCTAGGGCGATATCTTTGAGAGAGTTGCTTCTGAG880.5671199329767352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT830.5348972095121479No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAAT830.5348972095121479No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC710.45756267319713867No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT700.45111812850422117No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCT680.4382290391183863No Hit
ATTGCGCAATGTTGATGCTGGGGGCCGAGAGAGACCTTCGTACATGGGAC550.3544499581104595No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG510.3286717793387897No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCG500.3222272346458723No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT480.3093381452600374No Hit
ATTGCGCAATGACTGGGAGGGGAGTTTCCAAGGGCGCATGGTGGATGCCT410.26422633240961524No Hit
ATTGCGCAATGGCAGTTCGGGGCAGAAAAAACAAAACACAAACAAAAACC410.26422633240961524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG410.26422633240961524No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC400.2577817877166978No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT360.23200360894502803No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC360.23200360894502803No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA340.21911451955919314No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG330.2126699748662757No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG290.1868917960946059No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC280.18044725140168846No Hit
ATTGCGCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCA260.16755816201585358No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC250.16111361732293614No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCT250.16111361732293614No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTC250.16111361732293614No Hit
ATTGCGCAATGCTATGTTGGGGGGCACCACGACTAGTGTTCACACGACTG240.1546690726300187No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT240.1546690726300187No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC220.1417799832441838No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT220.1417799832441838No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC220.1417799832441838No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCGCG210.13533543855126634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT210.13533543855126634No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT210.13533543855126634No Hit
ATTGCGCAATGAGAACGCAGGGAAGTTCGAGAAGGCCCGTGGCCGCAGAA200.1288908938583489No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACACTCG200.1288908938583489No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG200.1288908938583489No Hit
ATTGCGCAATCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA180.11600180447251401No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGG170.10955725977959657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCT170.10955725977959657No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCGCACAATCTCG170.10955725977959657No Hit
ATTGCGCAATGATGCAAGAGGGGGAGTTACAAAAGAACATGTTAGACGAA170.10955725977959657No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT160.10311271508667913No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTG160.10311271508667913No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT160.10311271508667913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)