FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11505
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA7656.6492829204693615No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT5574.841373315949586No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3252.824858757062147No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.6171229900043459No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT540.469361147327249No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC520.4519774011299435No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTG460.39982616253802694No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA440.3824424163407214No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG420.3650586701434159No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.3476749239461104No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA370.32159930465015213No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG340.2955236853541938No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG330.28683181225554105No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC310.26944806605823557No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG280.24337244676227726No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTT270.2346805736636245No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA270.2346805736636245No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA260.22598870056497175No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC260.22598870056497175No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC260.22598870056497175Illumina RNA PCR Primer (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGA250.21729682746631898No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG230.19991308126901347No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCGATGGTTGTGTA230.19991308126901347No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA230.19991308126901347No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGGA220.1912212081703607No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGGTGTGTA200.1738374619730552No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGT190.16514558887440245No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCG180.15645371577574968No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.15645371577574968No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC170.1477618426770969No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT170.1477618426770969No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.13906996957844417No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC150.1303780964797914No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTGGCC150.1303780964797914Illumina Single End PCR Primer 1 (95% over 21bp)
GAGCCGAGGACCTCCTCCGGGATCCGCTCATCGTCGAGTGGGCCTGCGAG140.12168622338113863No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATGTCGG140.12168622338113863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA140.12168622338113863No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTCGG140.12168622338113863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC140.12168622338113863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC140.12168622338113863No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCAA130.11299435028248588No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA130.11299435028248588No Hit
GTATAAGAGACAGATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10430247718383312No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT120.10430247718383312No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGA120.10430247718383312No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCAA120.10430247718383312No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA120.10430247718383312No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT120.10430247718383312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)