FastQCFastQC Report
Wed 18 Jan 2023
EGAF00007822074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14037
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA9226.568355061622853No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6694.765975635819619No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4523.2200612666524187No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTG780.5556742893780722No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT760.5414262306760704No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.39894564365605184No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA550.3918216143050509No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC540.38469758495405004No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG530.37757355560304906No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.3419534088480444No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG480.3419534088480444No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG460.3277053501460426No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC400.28496117404003707No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGGA390.2778371446890361No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA360.25646505663603336No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC350.2493410272850324No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG340.2422169979340315No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTT330.23509296858303055No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC330.23509296858303055Illumina RNA PCR Primer (95% over 22bp)
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC320.22796893923202963No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA320.22796893923202963No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG310.2208449098810287No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGA300.21372088053002777No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCG250.17810073377502314No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA230.1638526750730213No Hit
TGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGT230.1638526750730213No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCGATGGTTGTGTA220.1567286457220204No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.14960461637101946No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTCGG210.14960461637101946No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC200.14248058702001853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC200.14248058702001853No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC200.14248058702001853No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA190.1353565576690176No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA180.12823252831801668No Hit
ATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGG170.12110849896701575No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA170.12110849896701575No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATA170.12110849896701575No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGGTGTGTA150.10686044026501389No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA150.10686044026501389No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTC150.10686044026501389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)