FastQCFastQC Report
Tue 7 May 2024
EGAF00007822079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12357
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG5074.1029376062151No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5014.054382131585337No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCT3262.6381807882172046No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC2752.2254592538642064No Hit
ATTGCGCAATGTGCGACAGGGGGGCGAAAGCCTGATCCAGCAATGCCGCG1641.3271829732135632No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.7121469612365461No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAT670.5422028000323703No Hit
ATTGCGCAATGAACATATCGGGGGGAAATGCAGTTAAGCCTGCAGTGGAC620.5017399045075666No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC580.4693695880877235No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTG540.43699927166788055No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG520.4208141134579591No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC480.38844379703811605No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG450.3641660597232338No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT440.35607348061827304No Hit
ATTGCGCAATGGATTTAACGGGGTGGTGATCCTGTACTGTCGAGAAAAGC410.3317957433033908No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT390.3156105850934693No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT370.2994254268835478No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC320.25896253135874403No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCG260.21040705672897955No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCT250.20231447762401877No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.20231447762401877No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT240.19422189851905802No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACC240.19422189851905802No Hit
ATTGCGCAATGAATGGCGGGGGGGCGAAAGCCTGATCCAGCAATGCCGCG240.19422189851905802No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC220.17803674030913652No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG200.16185158209921502No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCAGCG190.15375900299425427No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC180.14566642388929352No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG170.13757384478433277No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA160.12948126567937202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG160.12948126567937202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTG160.12948126567937202No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC150.12138868657441126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT150.12138868657441126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC150.12138868657441126No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCGCG150.12138868657441126No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT140.11329610746945051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC140.11329610746945051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTGT140.11329610746945051No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.11329610746945051No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTACCTATTGCATCTCG130.10520352836448978No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCT130.10520352836448978No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT130.10520352836448978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)