FastQCFastQC Report
Tue 7 May 2024
EGAF00007822080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12357
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA7736.25556364813466No Hit
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT6425.195435785384802No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC3512.8404952658412235No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC600.48555474629764506No Hit
CAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTG580.4693695880877235No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT560.45318442987780205No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.45318442987780205No Hit
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA520.4208141134579591No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG460.37225863882819454No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC460.37225863882819454No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC410.3317957433033908No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCA380.30751800598850854No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGGA330.2670551104637048No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.25896253135874403No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG310.2508699522537833No Hit
GGGTTGAGCCCCGGGCTTTCACCTCTGACTTAAAAAACCGCCTACGCGCG290.23468479404386175No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCG260.21040705672897955No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTT250.20231447762401877No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC250.20231447762401877Illumina RNA PCR Primer (95% over 22bp)
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA240.19422189851905802No Hit
ACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA240.19422189851905802No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG240.19422189851905802No Hit
GCATACATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGAT220.17803674030913652No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAA210.16994416120417577No Hit
TTTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCG210.16994416120417577No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA210.16994416120417577No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGC210.16994416120417577No Hit
GGCTTTCACCTCTGACTTAAAAAACCGCCTACGCGCGCTTTACGCCCAGT200.16185158209921502No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG190.15375900299425427No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAA190.15375900299425427No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGCA190.15375900299425427No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGC180.14566642388929352No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGC170.13757384478433277No Hit
ATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGG170.13757384478433277No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGAGTCGATGGTTGTGTA170.13757384478433277No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGTTCTCGG170.13757384478433277No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCA160.12948126567937202No Hit
GATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAT160.12948126567937202No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGC160.12948126567937202No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTGGGC140.11329610746945051No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGA140.11329610746945051No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGA130.10520352836448978No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAATAT130.10520352836448978No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGCAAT130.10520352836448978No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCG130.10520352836448978No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)