FastQCFastQC Report
Tue 7 May 2024
EGAF00007822081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7732
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC62

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC7149.234350750129334No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC3344.319710294878427No Hit
ATTGCGCAATGTTCACCGGGGGAGGTTAAGGGGACCCGTTAGCTTCGGCG580.7501293326435592No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG540.6983962752198655No Hit
ATTGCGCAATGGCGTGATGGGGCGCTGGCGGCGTGCTTAACACATGCAAG310.4009311950336265No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG280.3621314019658562No Hit
ATTGCGCAATGAGTGAGGCGGGCAAAACTAGCGGATGAGCTGTGGATAGG210.2715985514743921No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCG210.2715985514743921No Hit
ATTGCGCAATCTGTCTCTTATACACCGGTCTCTTATACACATCTCCGAGC200.2586652871184687No Hit
ATTGCGCAATCTGTCTCTTATACACCTGGCTCTTATACACATCTCCGAGC190.24573202276254527No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG180.23279875840662181No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.23279875840662181No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGAT170.21986549405069838No Hit
ATTGCGCATGCCAAGCAAAGTGATGTTCGTGCGCGTCTGTCGAGCGGCGA140.1810657009829281No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTAT140.1810657009829281No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGAT130.16813243662700464No Hit
GGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.16813243662700464No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT110.14226590791515778No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC100.12933264355923435No Hit
ATTGCGCAATGCGAAAGACGGGGAATTTACAATGGATCTTCGTACCAAGA90.11639937920331091No Hit
ATTGCGCAATGGAGCTTCCGGGGGATCGTAAAACTCTGTTATTAGGGAAG90.11639937920331091No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTGATACACATCTCCGAGC90.11639937920331091No Hit
ATTGCGCAAACTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC90.11639937920331091No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTGATGGTATGATCT80.10346611484738748No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGATG80.10346611484738748No Hit
ATTGCGCAATCTGTCTCTTATACCTGGCTCTTATACACATCTCCGAGCCC80.10346611484738748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)