FastQCFastQC Report
Tue 7 May 2024
EGAF00007822082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7732
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCT97012.545266425245732No Hit
GTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGC4786.182100362131402No Hit
GATGTGTATAAGAGACAGATTGCGCAATCTGTCTCTTATACACATCTGAC821.0605276771857217No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTA811.0475944128297983No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGCC390.5043973098810139Illumina RNA PCR Primer (95% over 22bp)
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGGGGGC180.23279875840662181No Hit
CTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGG150.19399896533885153No Hit
ATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGG140.1810657009829281No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTGGCC140.1810657009829281Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG130.16813243662700464No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTGGGC130.16813243662700464No Hit
CTCTTATACACATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTC120.1551991722710812No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGGGGTCGCC110.14226590791515778Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGGCGCC110.14226590791515778Illumina Single End PCR Primer 1 (95% over 21bp)
TTTTTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGA110.14226590791515778No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGGGGCC110.14226590791515778No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGGGGGGGGC100.12933264355923435No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTATATCTCGGTGGTCGCC100.12933264355923435Illumina Single End PCR Primer 1 (95% over 21bp)
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG100.12933264355923435No Hit
CATCTGACGCTGCCGACGATTCGATGGTTGTGTAGATCTCGGTGGTCGGC100.12933264355923435Illumina Single End PCR Primer 1 (95% over 21bp)
CTATAGTAAAGGTCACGGGGTCTTTCCGTCCTTCTGCGCGTAACGAGCAT80.10346611484738748No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGATGGTT80.10346611484738748No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGC80.10346611484738748No Hit
GAGACAGATTGCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGA80.10346611484738748No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATTCGAGGGTTGTGTA80.10346611484738748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)