FastQCFastQC Report
Tue 7 May 2024
EGAF00007822085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007822085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9877
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG4744.799028044952921No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT3423.4625898552191963No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC2612.6425027842462288No Hit
ATTGCGCAATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC1541.559177888022679No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAT610.6175964361648274No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.5872228409436063No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG490.4961020552799433No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC460.46572846005872226No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCG410.41510580135668723No Hit
ATTGCGCAATGTGGGATGGGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAG390.3948567378758733No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA380.38473220613546627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT370.3746076743950592No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT360.36448314265465226No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT350.3543586109142452No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCG280.28348688873139616No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGAGCG270.27336235699098915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC270.27336235699098915No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC240.24298876176976816No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA230.23286423002936113No Hit
ATTGCGCAATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCG210.21261516654854712No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT180.18224157132732613No Hit
ATTGCGCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCT170.17211703958691912No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT160.1619925078465121No Hit
ATTGCGCAATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAT150.15186797610610508No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTG140.14174344436569808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG140.14174344436569808No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG140.14174344436569808No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTA130.13161891262529107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT130.13161891262529107No Hit
ATTGCGCAATGCGCGTGTCGGGGGGCCTGCGTGTGTTCTCCGGCTACTTA130.13161891262529107No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCTCG130.13161891262529107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGGC130.13161891262529107No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG120.12149438088488408No Hit
ATTGCGCAATGTTTACGTGGGGTCAAGTTAAAAAGAGCACTAGGTGAATG120.12149438088488408No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC120.12149438088488408No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGT110.11136984914447708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTG110.11136984914447708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCGGTC110.11136984914447708No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATGG110.11136984914447708No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC100.10124531740407007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCCTGT100.10124531740407007No Hit
ATTGCGCAATGGTGGTTACGGGAGGACTGTGTGACCGATGCCGATCGGAC100.10124531740407007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTCTT100.10124531740407007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGCT100.10124531740407007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGC100.10124531740407007No Hit
ATTGCGCAATGACACTCATGGGGTAAGAAAGAGCACACGGGGGATGCCTA100.10124531740407007No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTGT100.10124531740407007No Hit
ATCTGTGTCTTATACACATCTCCGAGCCCACGAGACTCTATAGCGGATCT100.10124531740407007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)